7YO8

Crystal structure of fission yeast Hfl1 LIR fused to human GABARAPL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-24
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 43 21 2
Unit cell lengths	45.850, 45.850, 152.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.910 - 1.805
R-factor	0.1785
Rwork	0.177
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4co7
RMSD bond length	0.006
RMSD bond angle	0.774
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.19.2)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.910	43.910	1.910
High resolution limit [Å]	1.805	5.380	1.805
Rmerge	0.154	0.087	1.287
Rmeas	0.161	0.093	1.389
Rpim	0.045
Number of reflections	15809	1077	4612
<I/σ(I)>	11	19.02	1.52
Completeness [%]	99.9	99.9	99
Redundancy	12.7	7.277	6.972
CC(1/2)	0.997	0.995	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	8% PEG 3000, 0.1 M sodium citrate