4CO7

Crystal structure of human GATE-16

Summary for 4CO7

• Entry DOI: 10.2210/pdb4co7/pdb
• Descriptor: GAMMA-AMINOBUTYRIC ACID RECEPTOR-ASSOCIATED PROTEIN-LIKE 2 (2 entities in total)
• Functional Keywords: protein transport, autophagy, beta-grasp fold, ubiquitin superfamily
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Golgi apparatus : P60520
• Total number of polymer chains: 2
• Total formula weight: 27661.93

Authors

Weiergraeber, O.H.,Ma, P.,Willbold, D. (deposition date: 2014-01-27, release date: 2015-01-14, Last modification date: 2023-12-20)

Primary citation

Ma, P.,Schillinger, O.,Schwarten, M.,Lecher, J.,Hartmann, R.,Stoldt, M.,Mohrluder, J.,Olubiyi, O.,Strodel, B.,Willbold, D.,Weiergraber, O.H.
Conformational Polymorphism in Autophagy-Related Protein Gate-16.
Biochemistry, 54:5469-, 2015

PubMed Abstract: Autophagy is a fundamental homeostatic process in eukaryotic organisms, fulfilling essential roles in development and adaptation to stress. Among other factors, formation of autophagosomes critically depends on proteins of the Atg8 (autophagy-related protein 8) family, which are reversibly conjugated to membrane lipids. We have applied X-ray crystallography, nuclear magnetic resonance spectroscopy, and molecular dynamics simulations to study the conformational dynamics of Atg8-type proteins, using GATE-16 (Golgi-associated ATPase enhancer of 16 kDa), also known as GABARAPL2, as a model system. This combination of complementary approaches provides new insight into a structural transition centered on the C-terminus, which is crucial for the biological activity of these proteins.

PubMed: 26284781
DOI: 10.1021/ACS.BIOCHEM.5B00366

Experimental method

X-RAY DIFFRACTION (2 Å)