7YLF

Crystal structure of the chicken Toll-like receptor 15 TIR domain (2-mercaptoethanol adduct)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	64.299, 64.299, 95.782
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.840 - 1.900
R-factor	0.2029
Rwork	0.201
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nuw
RMSD bond length	0.007
RMSD bond angle	0.816
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	18382	902
<I/σ(I)>	32.9	2.3
Completeness [%]	98.7	98.8
Redundancy	5.4	5.5
CC(1/2)	0.996	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	Ammonium sulfate, NaCl, sodium cacodylate