7YIZ

Crystal structure of anti-mPEG h15-2b Fab W104Y mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	80
Detector technology	CCD
Collection date	2021-06-12
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.335, 112.056, 157.792
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 2.420
R-factor	0.2183
Rwork	0.216
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7y0g
RMSD bond length	0.006
RMSD bond angle	1.472
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.510
High resolution limit [Å]	2.420	2.420
Rmerge	0.060	0.360
Number of reflections	42950	3809
<I/σ(I)>	30.135	3.7
Completeness [%]	98.7	89.5
Redundancy	6.7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% (w/v) PEG-6000, 1% (w/v) PEG-2000-MME, 0.15 M lithium sulfate monohydrate, and 0.1 M citric acid