7YFJ
Crystal structure of human WTAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.754, 62.754, 336.345 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.640 - 2.400 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.490 |
| High resolution limit [Å] | 2.397 | 2.397 |
| Rmerge | 0.099 | 0.978 |
| Rmeas | 0.103 | |
| Rpim | 0.031 | 0.300 |
| Number of reflections | 16547 | 1679 |
| <I/σ(I)> | 19.8 | 4.6 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 20 | 20.7 |
| CC(1/2) | 0.998 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | MES, magnesium nitrate, sodium bromide, PEG6000, glycerol |
