7YD3

Single-chain variable fragment of app 3D1 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	77
Detector technology	CCD
Collection date	2021-10-04
Detector	MAR CCD 130 mm
Wavelength(s)	0.9785
Spacegroup name	P 1
Unit cell lengths	47.183, 70.490, 74.815
Unit cell angles	108.83, 93.82, 90.09

Refinement procedure

Resolution	19.920 - 2.160
R-factor	0.219
Rwork	0.217
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kvf
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.240
High resolution limit [Å]	2.160	2.160
Number of reflections	155752	45947
<I/σ(I)>	7.6
Completeness [%]	94.4
Redundancy	3.4
CC(1/2)	0.910	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% 2-propanol, 0.1 M HEPES pH 7.5, 20% PEG 4000