7YB7

anti-apoptotic protein BCL-2-M12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-01-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	104.761, 104.788, 111.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.520 - 2.200
R-factor	0.17626
Rwork	0.175
R-free	0.19375
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.961
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.200	6.100	2.250
Rmerge	0.104	0.062	0.432
Rmeas	0.107	0.064	0.442
Rpim	0.023	0.015	0.093
Number of reflections	29689	886	742
<I/σ(I)>	6.7
Completeness [%]	100.0	99.9	100
Redundancy	20	20.1	22.3
CC(1/2)	0.994	0.998	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.5 M Ammonium sulfate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Lithium sulfate monohydrate