7YAN
UDP-glucuronosyltransferase2B17 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-17 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.150, 57.308, 74.074 |
| Unit cell angles | 99.25, 94.72, 90.12 |
Refinement procedure
| Resolution | 19.110 - 2.500 |
| Rwork | 0.268 |
| R-free | 0.33080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ipb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.459 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.110 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | |
| Number of reflections | 20837 | 2019 |
| <I/σ(I)> | 8.05 | |
| Completeness [%] | 97.5 | |
| Redundancy | 2.7 | |
| CC(1/2) | 0.985 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 6000, 0.2M Na-Tartrate, 0.2M KCl, 2% glycerol |
