7Y9C
Crystal structure of METTL9 in complex with SLC39A5 peptide and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 106.280, 46.046, 124.780 |
Unit cell angles | 90.00, 103.35, 90.00 |
Refinement procedure
Resolution | 31.829 - 2.100 |
R-factor | 0.1945 |
Rwork | 0.192 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | model from alphafold2 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.050 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 34530 | 34530 |
<I/σ(I)> | 14.6 | |
Completeness [%] | 99.6 | |
Redundancy | 6.4 | |
CC(1/2) | 0.998 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MES monohydrate pH6.5, 12 % w/v PEG 20000, 0.2 M sodium thiocyanate |