7Y8K
Structure of ScIRED wild-type from Streptomyces clavuligerus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-28 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 48.195, 63.731, 162.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.440 - 2.080 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oqy |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.440 | 2.180 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.127 | 0.426 |
| Number of reflections | 57293 | 2112 |
| <I/σ(I)> | 8.25 | 2.27 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 7.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 12% Polyethylene glycol 8000, 0.1 M 2-(4-Morpholino) ethanesulfonic acid pH 6.0, 0.1M Calcium Acetate |
