7Y7P
QDE-1 in complex with RNA template, RNA primer and AMPNPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-03 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97928 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.949, 120.201, 114.857 |
Unit cell angles | 90.00, 109.62, 90.00 |
Refinement procedure
Resolution | 29.620 - 2.700 |
R-factor | 0.2242 |
Rwork | 0.223 |
R-free | 0.25420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j7n |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.800 | 2.700 |
Rmerge | 0.063 | 0.039 | 0.333 |
Rmeas | 0.070 | 0.043 | 0.390 |
Rpim | 0.029 | 0.016 | 0.196 |
Total number of observations | 337787 | ||
Number of reflections | 70175 | 7270 | 6751 |
<I/σ(I)> | 7.9 | ||
Completeness [%] | 98.1 | 99.8 | 94.4 |
Redundancy | 4.8 | 6.4 | 3.2 |
CC(1/2) | 0.999 | 0.714 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | PEG 4000, 200 mM KCl |