7Y5P
Crystal structure of CmnC in complex with L-arginine and alpha-KG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-06-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9732 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.998, 127.292, 139.019 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.280 - 1.700 |
R-factor | 0.1788 |
Rwork | 0.177 |
R-free | 0.20360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vgl |
RMSD bond length | 0.006 |
RMSD bond angle | 1.291 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.055 | 0.604 |
Number of reflections | 90120 | 8944 |
<I/σ(I)> | 27.56 | 2.42 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M Ammonium acetate, 0.1M Na citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 400 |