7Y5O
Crystal structure of human CAF-1 core complex in spacegroup P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.613, 176.092, 100.913 |
Unit cell angles | 90.00, 115.09, 90.00 |
Refinement procedure
Resolution | 26.980 - 3.570 |
R-factor | 0.2153 |
Rwork | 0.214 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7y5k |
RMSD bond length | 0.002 |
RMSD bond angle | 0.464 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.730 |
High resolution limit [Å] | 3.570 | 3.600 |
Rmerge | 0.110 | 0.584 |
Number of reflections | 56505 | 2062 |
<I/σ(I)> | 12 | |
Completeness [%] | 99.8 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 7% Tacsimate, pH 6.0, 18% (w/v) PEG 3350, 0.12 M lithium citrate and 0.13 M sodium tartrate |