7Y43

Crystal structure of the KAT6A WH domain and its bound double stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97930
Spacegroup name	P 43 21 2
Unit cell lengths	46.479, 46.479, 153.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.600 - 1.500
R-factor	0.2274
Rwork	0.227
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lui
RMSD bond length	0.006
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.097	1.290
Number of reflections	28018	1352
<I/σ(I)>	41.7
Completeness [%]	100.0
Redundancy	24.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M magnesium acetate tetrahydrate, 20% polyethylene glycol 3,350