7Y1I
Crystal Structure of TTR-G67R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 31 |
Unit cell lengths | 140.982, 140.982, 165.287 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.150 - 2.790 |
R-factor | 0.2745 |
Rwork | 0.272 |
R-free | 0.31390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n85 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.719 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.980 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.198 | 0.796 |
Rpim | 0.069 | 0.516 |
Number of reflections | 101651 | 4059 |
<I/σ(I)> | 9.7 | 1.56 |
Completeness [%] | 99.6 | 95.4 |
Redundancy | 10.5 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | AMMONIUM SULFATE, PEG3350 |