7XZN

Formate-tetrahydrofolate ligase from Peptostreptococcus anaerobius

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	215.870, 116.300, 107.380
Unit cell angles	90.00, 92.58, 90.00

Refinement procedure

Resolution	30.500 - 2.040
R-factor	0.2716
Rwork	0.272
R-free	0.28270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a4j
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	MoRDa
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.020	2.090
High resolution limit [Å]	2.040	2.040
Number of reflections	168145	12394
<I/σ(I)>	8
Completeness [%]	99.9	100
Redundancy	7	7.2
CC(1/2)	0.998	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.15M Cesium chloride, 15% w/v PEG 3350