7XWZ
Crystal structure of SARS-CoV-2 N-NTD and dsRNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18057 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.848, 39.119, 84.157 |
| Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
| Resolution | 47.977 - 2.250 |
| Rwork | 0.226 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ofz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.977 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.049 | 0.432 |
| Number of reflections | 16782 | 1511 |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Malonic Acid, Imidazole, Boric Acid, PEG 1500 |
