7XUB
Structure of G9a in complex with compound 10d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-19 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.571, 77.464, 68.111 |
Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
Resolution | 38.050 - 2.000 |
R-factor | 0.1796 |
Rwork | 0.178 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xua |
RMSD bond length | 0.006 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 40123 | 2004 |
<I/σ(I)> | 19.5 | 2.4 |
Completeness [%] | 100.0 | |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Bis-Tris propane (7.75), 0.2M Sodium Formate, 10% Ethylene Glycol, 26% PEG3350 |