7XQK
The Crystal Structure of CDK3 and CyclinE1 Complex from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.18078 |
Spacegroup name | P 63 |
Unit cell lengths | 154.154, 154.154, 76.675 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.500 - 2.250 |
Rwork | 0.160 |
R-free | 0.20240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eoj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.494 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.500 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.139 | 0.973 |
Number of reflections | 49467 | 4563 |
<I/σ(I)> | 15.9 | 2.7 |
Completeness [%] | 100.0 | |
Redundancy | 10.2 | |
CC(1/2) | 0.998 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.6M MgSO4, 0.1M MES pH6.5 |