7XOF

Crystal structure of Oryza sativa plastid glycyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	74.130, 130.940, 123.390
Unit cell angles	90.00, 101.88, 90.00

Refinement procedure

Resolution	65.470 - 2.560
R-factor	0.2119
Rwork	0.210
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f5w
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.470	65.470	2.630
High resolution limit [Å]	2.560	11.450	2.560
Rmerge	0.066	0.027	0.467
Rmeas	0.083	0.034	0.592
Rpim	0.049	0.020	0.359
Total number of observations	168193	1858	12681
Number of reflections	68702	756	5162
<I/σ(I)>	9.5	27.2	2.1
Completeness [%]	93.0	88.1	94.8
Redundancy	2.4	2.5	2.5
CC(1/2)	0.997	0.998	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	289	PEG 3350