7XMN
Structure of SARS-CoV-2 ORF8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 140.088, 146.693, 74.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.130 - 2.300 |
| R-factor | 0.2164 |
| Rwork | 0.215 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3set |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.310 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 33261 | 3311 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 96.8 | 99.2 |
| Redundancy | 4.4 | 4.5 |
| CC(1/2) | 0.989 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES pH6.1, 2%Polyethylene glycol 400, 2.1 M Ammonium sulfate |
