7XME

Structure of Influenza A virus polymerase basic protein 2 (PB2) with an azazindole derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-31
Detector	DECTRIS PILATUS3 X CdTe 1M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	176.791, 31.306, 114.552
Unit cell angles	90.00, 95.31, 90.00

Refinement procedure

Resolution	45.958 - 2.521
R-factor	0.2539
Rwork	0.250
R-free	0.32970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p1u
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.016	2.565
High resolution limit [Å]	2.521	2.521
Number of reflections	21874	1099
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)	0.993	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	Soudium,BIS-Tris propane pH 8.5,PEG3350