7XLI
Crystal structure of IsdB linker-NEAT2 bound to a nanobody (VHH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.321, 48.580, 140.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.700 |
R-factor | 0.21 |
Rwork | 0.209 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xld |
RMSD bond length | 0.014 |
RMSD bond angle | 1.780 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.068 | 0.828 |
Rpim | 0.025 | 0.325 |
Number of reflections | 31303 | 4429 |
<I/σ(I)> | 14.7 | 2.3 |
Completeness [%] | 99.8 | |
Redundancy | 8.4 | |
CC(1/2) | 0.998 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 3,350 0.1 M HEPES (pH 7.5) |