7XLD
Crystal structure of IsdH linker-NEAT3 bound to a nanobody (VHH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-22 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.130, 49.420, 53.940 |
Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
Resolution | 40.900 - 1.650 |
R-factor | 0.149 |
Rwork | 0.148 |
R-free | 0.19650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4grw 3vtm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.512 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.068 | 0.642 |
Rpim | 0.035 | 0.333 |
Number of reflections | 32081 | 4588 |
<I/σ(I)> | 13.4 | 2.1 |
Completeness [%] | 96.1 | 94.9 |
Redundancy | 4.7 | |
CC(1/2) | 0.999 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3,350 0.1 M HEPES (pH 7.5) |