7XFR

Crystal structure of WIPI2b in complex with the second site of ATG16L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	74.199, 80.630, 76.755
Unit cell angles	90.00, 97.33, 90.00

Refinement procedure

Resolution	24.990 - 1.760
R-factor	0.1659
Rwork	0.165
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ltg
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.590	1.790
High resolution limit [Å]	1.760	1.760
Rmeas	0.037	0.748
Number of reflections	83257	4440
<I/σ(I)>	24.71
Completeness [%]	93.7
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1 M MES monohydrate(pH 6.5), 12% w/v Polyethylene glycol 20000