7XEI

SARS-CoV-2-prototyped-RBD and CB6-092-Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	80.046, 112.197, 101.741
Unit cell angles	90.00, 100.90, 90.00

Refinement procedure

Resolution	49.950 - 2.760
R-factor	0.2176
Rwork	0.215
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c01
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.610	2.910
High resolution limit [Å]	2.760	2.760
Rmerge	0.074	0.422
Number of reflections	41517	4309
<I/σ(I)>	11.1	2.5
Completeness [%]	91.8
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	Ammonium nitrate, PEG 3350