7XEG

SARS-CoV-2-Beta-RBD and CB6-092-Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	80.248, 113.236, 102.098
Unit cell angles	90.00, 100.58, 90.00

Refinement procedure

Resolution	39.240 - 2.690
R-factor	0.1977
Rwork	0.195
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c01
RMSD bond length	0.004
RMSD bond angle	0.840
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.790
High resolution limit [Å]	2.690	2.690
Rmerge	0.189	0.863
Number of reflections	47674	3714
<I/σ(I)>	10
Completeness [%]	99.5
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	Tris pH 8.0, PEG 2000