7XE1
Crystal structure of LSD2 in complex with cis-4-Br-PCPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-13 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9195 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 144.121, 170.760, 202.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.750 - 2.070 |
R-factor | 0.166 |
Rwork | 0.164 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fwe |
RMSD bond length | 0.007 |
RMSD bond angle | 0.899 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.390 | 2.110 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 151257 | |
<I/σ(I)> | 21.4 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.4 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 320mM Ammonium citrate, 22% PEG 3350 |