7XD9

Crystal Structure of Dengue Virus serotype 2 (DENV2) Polymerase Elongation Complex (CTP Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	139.430, 154.580, 172.351
Unit cell angles	90.00, 94.68, 90.00

Refinement procedure

Resolution	48.840 - 2.580
R-factor	0.1926
Rwork	0.191
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k6m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.670
High resolution limit [Å]	2.580	5.560	2.580
Rmerge	0.044	0.023	0.495
Rmeas	0.052	0.027	0.594
Rpim	0.029	0.015	0.326
Total number of observations	750342
Number of reflections	230467	23246	22908
<I/σ(I)>	16
Completeness [%]	99.8	99.1	99.8
Redundancy	3.3	3.1	3.2
CC(1/2)		0.999	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	Sodium formate, Glycerol