7XC0

Crystal structure of Human RPTPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.979
Spacegroup name	P 32
Unit cell lengths	56.460, 56.460, 80.447
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.450 - 1.560
R-factor	0.1796
Rwork	0.178
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nz6
RMSD bond length	0.003
RMSD bond angle	0.599
Data reduction software	HKL-2000 (v715)
Data scaling software	HKL-2000 (v715)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.450	1.616
High resolution limit [Å]	1.560	1.560
Rmerge	0.069	0.317
Number of reflections	38471	3574
<I/σ(I)>	10.6	2.89
Completeness [%]	94.2	87.66
Redundancy	3.9	2.5
CC(1/2)	0.997	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES-NaOH pH 6.5, 13%(v/v) PEGMME 550