7XB0

Crystal structure of Omicron BA.2 RBD complexed with hACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-24
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	103.261, 103.261, 224.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.660 - 2.900
R-factor	0.2176
Rwork	0.216
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.003
RMSD bond angle	0.535
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	26827	2649
<I/σ(I)>	13.3
Completeness [%]	96.5
Redundancy	8.8
CC(1/2)	0.988	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M ammonium acetate, 0.1 M Bis-Tris