7XAC

Dimeric structure of human galectin-7 in complex with two glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	53.596, 64.984, 71.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 1.800
R-factor	0.2048
Rwork	0.200
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.850	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.033	0.001
Number of reflections	23618	1388
<I/σ(I)>	18.6
Completeness [%]	99.7
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.2M Magnesium chloride, 0.1M Hepes pH7.5