7XAC
Dimeric structure of human galectin-7 in complex with two glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.596, 64.984, 71.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 1.800 |
R-factor | 0.2048 |
Rwork | 0.200 |
R-free | 0.25270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bkz |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.850 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.033 | 0.001 |
Number of reflections | 23618 | 1388 |
<I/σ(I)> | 18.6 | |
Completeness [%] | 99.7 | |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2M Magnesium chloride, 0.1M Hepes pH7.5 |