7X9G

Crystal structure of human EDA and EDAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	H 3
Unit cell lengths	87.169, 87.169, 156.354
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.710 - 2.800
R-factor	0.2493
Rwork	0.248
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rj7
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.044	0.034	0.696
Rmeas	0.052	0.041	0.824
Rpim	0.027	0.021	0.437
Total number of observations	37897
Number of reflections	10861	1087	1123
<I/σ(I)>	11.4
Completeness [%]	99.8	99.6	99.8
Redundancy	3.5	3.4	3.4
CC(1/2)		0.997	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	0.1M Bicine, pH9.0, 25% PEG 6000