7X8P
Frizzled 2 CRD in complex with pF7_A5 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 66.376, 66.661, 90.912 |
Unit cell angles | 95.14, 109.15, 115.33 |
Refinement procedure
Resolution | 27.760 - 2.240 |
R-factor | 0.2168 |
Rwork | 0.215 |
R-free | 0.24140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6c0b |
RMSD bond length | 0.007 |
RMSD bond angle | 0.969 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.760 | 2.290 |
High resolution limit [Å] | 2.240 | 2.250 |
Rmerge | 0.092 | 0.493 |
Rmeas | 0.109 | 0.597 |
Rpim | 0.058 | 0.331 |
Number of reflections | 59980 | 2991 |
<I/σ(I)> | 14.5 | |
Completeness [%] | 98.4 | 96.1 |
Redundancy | 3.5 | 3.1 |
CC(1/2) | 0.975 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 8% TacsimateTM, pH 4.0 and 20% PEG 3350 |