7X8P

Frizzled 2 CRD in complex with pF7_A5 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	66.376, 66.661, 90.912
Unit cell angles	95.14, 109.15, 115.33

Refinement procedure

Resolution	27.760 - 2.240
R-factor	0.2168
Rwork	0.215
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c0b
RMSD bond length	0.007
RMSD bond angle	0.969
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.760	2.290
High resolution limit [Å]	2.240	2.250
Rmerge	0.092	0.493
Rmeas	0.109	0.597
Rpim	0.058	0.331
Number of reflections	59980	2991
<I/σ(I)>	14.5
Completeness [%]	98.4	96.1
Redundancy	3.5	3.1
CC(1/2)	0.975	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	8% TacsimateTM, pH 4.0 and 20% PEG 3350