7X8J

Arabidopsis GDP-D-mannose pyrophosphorylase (VTC1) structure (unliganded)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	44.519, 146.862, 70.860
Unit cell angles	90.00, 100.46, 90.00

Refinement procedure

Resolution	32.633 - 2.798
R-factor	0.2169
Rwork	0.215
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d72
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.798	2.800
Rmerge	0.078	0.523
Rpim	0.047	0.310
Number of reflections	21924	2165
<I/σ(I)>	16.1	2.3
Completeness [%]	99.6	99.7
Redundancy	3.8	3.8
CC(1/2)	0.967	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	bis-tris, PEG 3350, lithium sulfate