7X2M

Crystal structure of nanobody 1-2C7 with SARS-CoV-2 RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	I 2 3
Unit cell lengths	143.619, 143.619, 143.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.390 - 1.800
R-factor	0.1809
Rwork	0.180
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.390	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	45687	4537
<I/σ(I)>	33.27	1.57
Completeness [%]	99.9
Redundancy	38.8
CC(1/2)	0.998	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2M Ammonium sulfate, 0.1M Bis Tris pH 5.5, 25% w/v Polyethylene glycol 3350