7X1K

Crystal structure of the flagellar expression regulator DegU from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-09-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	57.242, 57.242, 145.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.480 - 2.390
R-factor	0.2281
Rwork	0.227
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wsz
RMSD bond length	0.008
RMSD bond angle	0.935
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.390	2.390
Number of reflections	10058	480
<I/σ(I)>	29.5	1.2
Completeness [%]	98.1	99.2
Redundancy	6.9	6.7
CC(1/2)	0.998	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 3350, Tris