7WX1

E40K/M117L variant of Cu/Zn-superoxide dismutase from dog (Canis familiaris)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.275
Spacegroup name	P 21 21 21
Unit cell lengths	37.511, 50.170, 185.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.480 - 1.650
R-factor	0.2324
Rwork	0.231
R-free	0.26201
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.522
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	1.740
High resolution limit [Å]	1.650	1.650
Number of reflections	43243	6108
<I/σ(I)>	18.5
Completeness [%]	99.8
Redundancy	18.8
CC(1/2)	1.000	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.2M Sodium malonate, pH 7.0