7WWQ

Crystal structure of human Ufd1-Npl4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	F 2 2 2
Unit cell lengths	152.958, 165.424, 192.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.240 - 2.720
R-factor	0.2426
Rwork	0.241
R-free	0.27690
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.125
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.240	2.819
High resolution limit [Å]	2.700	2.722
Number of reflections	32527	2966
<I/σ(I)>	40.3	6.5
Completeness [%]	100.0
Redundancy	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1M Tris-HCl (pH8.5), 5 % (v/v) glycerol, and 1.3 M magnesium sulfate hydrate