7WWG
Crystal structure of Saccharomyces cerevisiae Sfh2 complexed with phosphatidylinositol in an open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2020-10-30 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.137, 75.137, 146.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.440 - 3.400 |
R-factor | 0.2447 |
Rwork | 0.240 |
R-free | 0.28250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.403 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.106 | 0.205 |
Rpim | 0.028 | |
Number of reflections | 10956 | 288 |
<I/σ(I)> | 48.8 | 5.5 |
Completeness [%] | 99.5 | 100 |
Redundancy | 14.8 | 15 |
CC(1/2) | 0.993 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M MES-NaOH pH 6.0, 30% PEG 1000 |