7WUA
Crystal structures of FadD32 from Corynebacterium diphtheriae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9783 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 108.647, 97.535, 142.043 |
Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
Resolution | 46.099 - 1.999 |
R-factor | 0.1868 |
Rwork | 0.185 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hm3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 1.999 | 5.430 | 2.000 |
Rmerge | 0.110 | 0.081 | 0.644 |
Rmeas | 0.124 | 0.092 | 0.725 |
Rpim | 0.056 | 0.042 | 0.330 |
Number of reflections | 198544 | 10140 | 9827 |
<I/σ(I)> | 5.4 | ||
Completeness [%] | 99.9 | 99.5 | 99.7 |
Redundancy | 4.7 | 4.6 | 4.7 |
CC(1/2) | 0.991 | 0.735 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 293 | 0.1 M NaCl, 0.1 M Bis-Tris pH 6.5, 1.5 M (NH4)2SO4 , VAPOR DIFFUSION, Sitting DROP, temperature 293K |