7WSZ
human glyoxalase I (with C-ter His tag) in glycerol-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.225, 96.995, 46.264 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.520 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vw9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.707 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.600 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.044 | 0.401 |
| Number of reflections | 56869 | |
| <I/σ(I)> | 18.7 | 2.2 |
| Completeness [%] | 95.4 | 71.6 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MIB (sodium malonate, imidazole, and boric acid), 25% PEG1500 |
