7WSZ
human glyoxalase I (with C-ter His tag) in glycerol-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2020-10-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.225, 96.995, 46.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.520 |
R-factor | 0.164 |
Rwork | 0.161 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vw9 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.707 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.600 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.044 | 0.401 |
Number of reflections | 56869 | |
<I/σ(I)> | 18.7 | 2.2 |
Completeness [%] | 95.4 | 71.6 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MIB (sodium malonate, imidazole, and boric acid), 25% PEG1500 |