7WPT
Methionyl-tRNA synthetase from Staphylococcus aureus complexed with a fragment M2-80 and ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-01 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.993, 76.536, 118.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.310 - 2.260 |
| R-factor | 0.2236 |
| Rwork | 0.221 |
| R-free | 0.26730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wpm |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.213 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.310 | 2.409 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.070 | 0.540 |
| Number of reflections | 24773 | 4141 |
| <I/σ(I)> | 12.2 | 3 |
| Completeness [%] | 93.2 | 98.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | Sodium cacodylate, PEG 3350 |
