7WPJ
Methionyl-tRNA synthetase from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-05 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.728, 68.987, 117.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.480 - 2.650 |
| R-factor | 0.2201 |
| Rwork | 0.217 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qre |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.730 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.030 | 0.070 |
| Number of reflections | 14046 | 1402 |
| <I/σ(I)> | 17.8 | 8.7 |
| Completeness [%] | 96.0 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | sodium cacodylate, PEG 3350 |
