7WL6

Crystal structure of I73L mutated human transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-21
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	42.840, 85.840, 64.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.674 - 1.418
R-factor	0.185636914987
Rwork	0.184
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n85
RMSD bond length	0.007
RMSD bond angle	1.048
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.700	1.490
High resolution limit [Å]	1.418	1.418
Rmerge	0.085	0.471
Rmeas	0.092	0.512
Rpim	0.035	0.197
Number of reflections	45459	6529
<I/σ(I)>	13.3	3.5
Completeness [%]	99.7
Redundancy	7
CC(1/2)	0.996	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	24% PEG400, 0.2 M magnesium acetate, 5% glycerol, 0.1 M Tris-HCl