7WJH

Crystal structure of Bcl-xL bound to the BH3 domain of human Pxt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2019-12-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	56.658, 60.675, 97.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.612 - 1.698
R-factor	0.1751
Rwork	0.172
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bzw
RMSD bond length	0.006
RMSD bond angle	0.750
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.698	1.698
Rmerge	0.076
Number of reflections	18530	948
<I/σ(I)>	33.7
Completeness [%]	97.5
Redundancy	4.7
CC(1/2)		0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M ammonium sulfate and 30% (w/v) polyethylene glycol 8,000