7WF6
Crystal structure of SNX13 RGS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 165.328, 165.328, 165.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 3.250 |
| R-factor | 0.2188 |
| Rwork | 0.218 |
| R-free | 0.22410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c7k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.393 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.230 | 29.230 | 3.510 |
| High resolution limit [Å] | 3.250 | 8.590 | 3.250 |
| Rmerge | 0.383 | 0.232 | 1.142 |
| Rmeas | 0.389 | 0.235 | 1.157 |
| Rpim | 0.061 | 0.041 | 0.182 |
| Total number of observations | 516745 | 22529 | 101270 |
| Number of reflections | 12744 | 780 | 2538 |
| <I/σ(I)> | 9.2 | 14.4 | 4 |
| Completeness [%] | 99.9 | 97.6 | 100 |
| Redundancy | 40.5 | 28.9 | 39.9 |
| CC(1/2) | 0.992 | 0.988 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | 0.7 M Sodium citrate tribasic, 0.1 M TRIS pH 8.5 |
