7WCX
Crystal structure of FGFR4(V550M) kinase domain with 7v
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-10-08 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5870 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.964, 61.342, 60.813 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 42.971 - 2.175 |
| R-factor | 0.1726 |
| Rwork | 0.170 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wct |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.741 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.971 | 2.253 |
| High resolution limit [Å] | 2.175 | 2.175 |
| Rmerge | 0.078 | 0.220 |
| Rmeas | 0.085 | |
| Number of reflections | 14797 | 1036 |
| <I/σ(I)> | 18.67 | 5.71 |
| Completeness [%] | 91.4 | 64.11 |
| Redundancy | 6.3 | 3.9 |
| CC(1/2) | 0.998 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350 |
