7WCT

Crystal structure of FGFR4 kinase domain with 7v

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-10-01
Detector	RIGAKU
Wavelength(s)	1.5870
Spacegroup name	P 1 21 1
Unit cell lengths	41.990, 61.438, 60.882
Unit cell angles	90.00, 98.51, 90.00

Refinement procedure

Resolution	43.003 - 2.106
R-factor	0.1838
Rwork	0.182
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jpj
RMSD bond length	0.003
RMSD bond angle	0.725
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.003	2.181
High resolution limit [Å]	2.106	2.106
Rmerge	0.074	0.210
Rmeas	0.080
Number of reflections	16660	1148
<I/σ(I)>	18.16	5.79
Completeness [%]	93.2	64.06
Redundancy	6.2	4
CC(1/2)	0.999	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350