7WC6
Crystal structure of serotonin 2A receptor in complex with LSD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 49.580, 54.450, 178.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.780 - 2.600 |
| R-factor | 0.242 |
| Rwork | 0.241 |
| R-free | 0.26290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a93 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.495 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.780 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 15628 | 1860 |
| <I/σ(I)> | 8.5 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.2 | 12.1 |
| CC(1/2) | 0.994 | 0.338 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293.15 | 100 mM Tris-HCl, 40 mM Potassium formate, 30% (v/v) PEG400, 2% (v/v) Polypropylene glycol P 400 |
